All clinical strains were genotypically characterized with the Geno Type assay.
In addition, for 67 of 79 INH- and/or RIF-resistant strains, genotypic characterization by sequencing was also available.
The overall concordance between pyrosequencing and the Geno Type MTBDRplus assay for clinical strains was 99.1%, and for clinical samples, it was 98.2%. The overall concordance between pyrosequencing and the Geno Type MTBDR strains is usually performed by time-consuming phenotypic methods.Commercial methods were performed following the manufacturer's instructions.).The Ziehl-Neelsen smears were graded on a scale from 0 to 4 (15).The susceptibility pattern of the isolated strains was characterized using Bactec 460TB.All strains were genotypically characterized using the Geno Type MTBDRDistribution of clinical specimens according to the Zielh-Neelsen staining and Bactec 460TB results.